FAQ

• Why is there noise on my images?
• How to optimally split (by wavelength) fluorescence between detectors?
• How to choose the pixel size?
• What is the optimal laser intensity?
• What is HV?
• How does a detector – a photomultiplier tube – work?
• How fast should I acquire my images?
• Should I image in 3D or 2D?
• What to do if you can’t see anything on your images?
• How to choose scanning speed/pixel exposure time?
• Why does fluorescence intensity decreases while I am imaging?
• What is the difference between unidirectional and bi-directional scanning?
• When and how to use the objective’s correction collar?
• How to choose laser excitation wavelength?
• What is signal-to-noise ratio?